Abstract

This document specifies a method for the enumeration of intestinal enterococci in water. The method is based on the growth of target organisms in a liquid medium and calculation of the “Most Probable Number” (MPN) of microorganisms by reference to MPN tables or using informatic programs. The test described in this document relies upon the detection of intestinal enterococci based upon expression of the enzyme ß-D-glucosidase. This method provides a confirmed result in 24 h with no requirement for confirmation of positive wells. This method can be applied to a range of types of water (for example, drinking water, bathing water, groundwater, surface water and wastewater), including those containing an appreciable amount of suspended matter and high background counts of heterotrophic bacteria. For marine waters, enterococci are best enumerated using diluted samples.

Begin

2023-07-14

WI

00230436

Planned document number

DIN EN ISO 7899-3

Project number

11904903

Responsible national committee

NA 119-01-03-03 UA - Microbiology  

Responsible european committee

CEN/TC 230 - Water analysis  

Responsible international committee

ISO/TC 147/SC 4/WG 29 - Enterococci  

draft standard

Water quality - Enumeration of intestinal enterococci - Part 3: Most probable number method (ISO/DIS 7899-3:2024); German and English version prEN ISO 7899-3:2024
2024-06
Order from DIN Media