Abstract

This standard describes the detection and isolation of Shiga toxin-producing Escherichia coli (STEC). The procedure includes the detection by Real Time PCR of the major virulence genes of STEC, stx1 and stx2 (Reference [2]) in enrichment cultures. Isolation of STEC from the enrichment culture is attempted if one or both stx genes are detected in the screening. This document is applicable to - products intended for human consumption, - products intended for animal feeding, - environmental samples in the area of food and feed production, handling, and - samples from the primary production stage.

Begin

2022-08-15

WI

00463108

Planned document number

DIN EN ISO 13136-1

Project number

05702629

Responsible national committee

NA 057-01-06 AA - Microbiology of the food chain  

Responsible european committee

CEN/TC 463/WG 2 - Shiga toxin producing Escherichia coli (STEC)  

Responsible international committee

ISO/TC 34/SC 9/WG 38 - Shiga-toxin producing Escherichia coli (STEC)  

draft standard

Microbiology of the food chain - Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) - Part 1: Horizontal method for the detection and isolation of Shiga toxin-producing Escherichia coli (STEC) (ISO/DIS 13136-1:2024); German and English version prEN ISO 13136-1:2024
2024-03
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previous edition(s)

Microbiology of food and animal feed - Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens - Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups (ISO/TS 13136:2012); German version CEN ISO/TS 13136:2012
2013-04

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