• Hydrogen Technologies Standards form the basic framework for market ramp-up

    More information
  • Climate change Standards and specifications support climate targets

    More information
  • Smart Farming Standards and specifications are drivers for the digitalization of agriculture

    More information
Standards [CURRENT]

DIN EN 12823-1
Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 1: Measurement of all-E-retinol and 13-Z-retinol; German version EN 12823-1:2014

Title (German)

Lebensmittel - Bestimmung von Vitamin A mit Hochleistungs-Flüssigchromatographie - Teil 1: Bestimmung von all-E-Retinol und 13-Z-Retinol; Deutsche Fassung EN 12823-1:2014

Overview

This European Standard specifies a method for the determination of vitamin A in foodstuffs by high performance liquid chromatography (HPLC). This determination has been validated in an interlaboratory study with samples of margarine and milk powder with all-E-retinol levels ranging from 653 µg/100 g to 729 µg/100 g and with 13-Z-retinol levels ranging from 30 µg/100 g to 39 µg/100 g. The determination of vitamin A content is carried out by the measurement of all-E-retinol, 13-Z-retinol and β-carotene. This part covers the measurement of all-E-retinol and 13-Z-retinol. The extract obtained in this method, after the saponification can be used for the determination of β-carotene, as described in EN 12823-2:2000 "Foodstuffs - Determination of vitamin A by high performance liquid chromatography - Part 2: Measurements of β-carotene". In this case, the saponification temperature should preferably not exceed 80 °C in order to prevent isomerization and oxidation of β-carotene. In this standard, retinol is released by saponification by using methanolic or ethanolic potassium hydroxide solution; then retinol is extracted by an appropriate solvent and determined by high performance liquid chromatography (HPLC) with either fluorescence (F) or ultraviolet (UV) detection. The substances are identified on the basis of their retention times and determined by the external standard procedure using peak areas or heights. This document has been prepared by Technical Committee CEN/TC 275 "Food analysis - Horizontal methods", the secretariat of which is held by DIN (Germany). The responsible German body is Working Committee NA 057-01-13 AA "Vitamine und Carotinoide" ("Vitamins and carotinoids") at the Food and Agricultural Products Standards Committee (NAL) at DIN.

Document: references other documents

Responsible national committee

NA 057-01-13 AA - Vitamins and carotinoids  

Responsible european committee

CEN/TC 275/WG 9 - Vitamins and Carotenoids  

Edition 2014-08
Original language German
Translation English
Price from 77.90 €
Table of contents

Contact

Dr.

Sophie Oberländer-Hayn

Am DIN-Platz, Burggrafenstr. 6
10787 Berlin

Tel.: +49 30 2601-2650
Fax: +49 30 2601-42650

Send message to contact