DIN EN 15950
Fertilizers - Determination of N-(1,2-dicarboxyethyl)-D,L-aspartic acid (Iminodisuccinic acid, IDHA) using high-performance liquid chromatography (HPLC); German version EN 15950:2010
Düngemittel - Bestimmung von N-(1,2-Dicarboxyethyl)-D,L-asparaginsäure (Iminodibernsteinsäure, IDHA) mit Hochleistungs-Flüssigchromatographie (HPLC); Deutsche Fassung EN 15950:2010
Overview
The European Standard has been prepared under the mandate M/418 under German project management of LUFA Kassel. The standard specifies a method for the determination of N-(1,2-dicarboxyethyl)-D,L-aspartic acid (iminodisuccinic acid, IDHA) in fertilizers. IDHA is measured and detected by HPLC after total conversion of the chelating agent into its iron-(III)-salts by adding an excess of a solution of iron-(III)-nitrate. In the HPLC chromatogram IDHA is represented by two dominant peaks. The first represents the R,S-isomer (50 %), and the second the R,R- as well as the S,S-isomer (each 25 %). The method is applicable to all fertilizers containing IDHA as chelating agent. The European Standard has been prepared by CEN/TC 260 "Fertilizers and liming materials" (secretariat: DIN). National Mirror Committee is Working Committee NA 057-03-02 AA "Düngemittel" ("Fertilizers") of NAL.
