Overview

This standard specifies a method for the quantitative determination of phospholipids by high performance liquid chromatography (HPLC) using a diol column and a light-scattering detector. The method is applicable to crude, oil-containing lecithins and to oil-free lecithins and lecithin fractions from vegetable fats and oils. The method is not applicable to animal and ruminant lecithins and enzymatically hydrolyzed lecithins; the peak separation of lysophosphytidylethanolamine (LPE), lysophosphatidylinositol (LPI) and lysophosphatidic acid (LPA) is insufficient. The International Standard has been prepared by ISO/TC 34/SC 11 "Animal and vegetable fats and oils" (secretariat: BSI, United Kingdom). CEN/TC 307 "Oilseeds, vegetable and animal fats and oils and their by-products - Sampling and test methods" (secretariat: AFNOR, France) is responsible for the European Standard. On the national level "Gemeinschaftsausschuss für die Analytik von Fetten, Ölen, Fettprodukten, verwandten Stoffen und Rohstoffen" (GA Fett) ("Joint Committee for the analysis of fats, oil, fat products, related substances and raw materials") of German Society for Fat Science (DGF) and NAL are responsible for the standard.

Document: references other documents

Responsible national committee

NA 057-05-05 AA - Joint committee of DIN and DGF for the analysis of fats, oils and products thereof, related and primary products  

Responsible european committee

CEN/TC 307 - Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis  

Responsible international committee

ISO/TC 34/SC 11 - Animal and vegetable fats and oils