DIN Standards Committee Food and Agricultural Products
DIN CEN ISO/TS 18867
; DIN SPEC 10056:2016-01
Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis (ISO/TS 18867:2015); German version CEN ISO/TS 18867:2015
Mikrobiologie der Lebensmittelkette - Polymerase-Kettenreaktion (PCR) zum Nachweis von pathogenen Mikroorganismen in Lebensmitteln - Nachweis von pathogenen Yersinia enterocolitica und Yersinia pseudotuberculosis (ISO/TS 18867:2015); Deutsche Fassung CEN ISO/TS 18867:2015
Procedure
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Overview
This Technical Specification is a test standard and specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCRbased methods. The described methods allow for the detection of the two pathogens in enrichments and allow the isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plague harbours a variant of the ail gene as well and will be detected by the same primer/probe set as Y. pseudotuberculosis. However, Y. pestis is normally not associated with food. This Technical Specification is applicable to products for human consumption, animal feeding stuffs, and environmental samples. It is intended for consumer health protection. The committee responsible for this standard is NA 057-01-06-01 AK "Polymerase-Kettenreaktion zum Nachweis von Mikroorganismen" ("Polymerase chain reaction for the detection of microorganisms") at DIN.
Document: references other documents
Responsible national committee
NA 057-01-06 AA - Microbiology of the food chain
Responsible european committee
CEN/TC 463/WG 1 - General requirements relating to PCR methods
