DIN Standards Committee Food and Agricultural Products
DIN EN 16160
Animal feeding stuffs - Determination of Hydrocyanic acid by HPLC; German version EN 16160:2012
Futtermittel - Bestimmung von Blausäure mittels HPLC; Deutsche Fassung EN 16160:2012
Overview
The standard DIN EN 16160 is applicable to the quantitative analysis of (bound and free) hydrocyanic acid (HCN) in feed materials of plant origin and compound feed by High Performance Liquid Chromatography (HPLC). The method is validated from 10 mg HCN/kg to 350 mg HCN/kg. When the method is used outside this range it should be validated at least within the laboratory. A limit of quantification of 2 mg HCN/kg should normally be obtained. Hydrocyanic acid occurs in feed as cyanoglycosides. Cyanoglycosides are degraded by enzymes generating cyanide. The European Standard has been prepared by CEN/TC 327 "Animal feeding stuffs - Methods of sampling and analysis", the secretariat of which is held by NEN (Netherlands). The collaboration of DIN for the field of responsibility of CEN/TC 327 is performed through national mirror committee, Working Committee NA 057-03-03 AA "Futtermittel" ("Animal feeding stuffs") at NAL.
Document: references other documents
Responsible national committee
NA 057-03-03 AA - Animal feeding stuffs