DIN Standards Committee Food and Agricultural Products
DIN EN ISO 22119
Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22119:2011); German version EN ISO 22119:2011
Mikrobiologie von Lebensmitteln und Futtermitteln - Real-time-Polymerase-Kettenreaktion (PCR) zum Nachweis von pathogenen Mikroorganismen in Lebensmitteln - Allgemeine Anforderungen und Begriffe (ISO 22119:2011); Deutsche Fassung EN ISO 22119:2011
Overview
The standard is applicable to the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). This standard describes requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real-time PCR. The minimum requirements laid down in this standard provide the basis for comparable and reproducible results in individual and between different laboratories. The standard has been prepared by Working Group "Polymerase-Kettenreaktion zum Nachweis von Mikroorganismen" ("Polymerase chain reaction for the detection of microorganisms") of Working Committee NA 057-01-06 AA "Mikrobiologische Lebensmitteluntersuchung einschließlich Schnellverfahren" ("Microbiological analysis of foodstuffs including rapid method") of NAL.
Document: references other documents
Responsible national committee
NA 057-01-06 AA - Microbiology of the food chain
Responsible european committee
CEN/TC 463/WG 1 - General requirements relating to PCR methods
